The Use of Probiotics to Fight Biofilms in Medical Devices


From 09:00 to 10:00

Instructor: Luciana Gomes / Fabio Carvalho

Biofilm-related infections are a critical problem in the medical field due to the high propensity of medical devices to microbial colonization and the low efficacy of the human immune system and antibiotics to counteract biofilm development. New evidence supporting the use of probiotics and their metabolites to control pathogenic biofilms and/or prevent microbial adhesion to several materials, and the increasing interest in promoting a natural approach to health have intensified the research in this field. Therefore, the interactions of two probiotic strains (Lactobacillus plantarum and Lactobacillus rhamnosus) with bacteria commonly found in biofilms developed in urinary tract devices (Escherichia coli and Staphylococcus aureus) were studied. Biofilm formation was carried out using microtiter plates and parallel-plate flow chambers (PPFC), and colony-forming unit (CFU) counts and crystal violet (CV) staining method were used to determine biofilm cell culturability and total biomass, respectively. The study showed that probiotics caused a reduction of pathogen adhesion up to 93%, suggesting the use of probiotics as potential antibiofilm agents for clinical applications.

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Unsupervised Machine Learning for Gene Expression Analysis


From 09:30 to 11:30

Instructor: Pedro Ferreira

In this talk the following topics will be discussed: - unsupervised machine learning as part of the exploratory data analysis process; - application of dimensionality reduction techniques to high dimensional datasets; - clustering to gain novel insights in the natural groups found in the data; - examples on different datasets will be shown.

Limit: 50 people

Towards the enhancement of human capabilities and mental well-being on athletes


From 10:00 to 11:30

Instructor: Hugo Ferreira

Find your mental powers with Neroes! Data is power. If you had the chance to collect and look at your own data, you could learn a thing or two about yourself and grow. How much would you like to learn about your mental data? If you replied "A lot!", then, in this workshop with Neroes, we will tell you how your mental abilities are. Learn about how you process information, how good you are at planning, and whether you're more of the attentive or impulsive type, among other things. Learn then how you can improve those mental skills and unleash your full potential! Don't you worry, there are no good or bad results, just how much you can improve! Join us and become your personal Hero!

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Molecular Gastronomy


From 10:00 to 11:30

Instructor: Ines Gomes

Our kitchens are laboratories, and chemistry is the basis of successful dishes. Molecular gastronomy uses the knowledge of physics and chemistry to study the processes that occur while cooking. This knowledge allows us to create new and innovative recipes. During this workshop special attention will be given to jellification process, namely spherification and reverse spherification as well as agar jellification will be presented. These techniques will allow to prepare fruit caviar, fruit egg yolk and mango spaghetti. The role of some jellifying ingredients and their importance for food industry will be discussed, as well as the importance of knowing the ingredients, and possible limitations that they can represent in the final result. The resolution of specific challenges will be asked and take-home experiences will be proposed.

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Deep Learning from Biomedical Data


From 11:30 to 13:00

Instructor: Joao Ribeiro Pinto

Deep learning techniques arose to take advantage of today's unprecedented amount of data collected from everything and everyone: sophisticated models are able to learn from raw data, identifying the most meaningful patterns and characteristics and using them for accurate and robust predictions. From character recognition to tumour diagnosis, deep learning has been pushing the boundaries of pattern recognition and helping companies and professionals in a wide array of fields. This workshop aims to prepare participants for advanced projects on the vibrant fields of pattern recognition and computer vision, delivering a solid foundation on deep learning through the introduction of essential theoretical concepts alongside a step-by-step practical tutorial on an example application to biomedical data.

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Alginate-based Biomaterials for Regenerative Medicine


From 11:30 to 13:00

Instructor: Silvia Bidarra

As the field of tissue engineering progressed, improvements in the fabrication of more refined and tailored cell based therapies are emerging to replace, repair or enhance the function of damaged tissues or organs. The success of cell delivery systems is dependent on the use of a biomaterial vehicle/scaffold that should allow controlling three-dimensional shape, guide tissue development and cell delivery into the patient. Therefore, such biomaterials should allow adhesion, spatial distribution, protection and guidance of cells. For clinical applications, cell delivery through a minimally invasive approach may be a desired method, since these systems offer specific advantages over preformed scaffolds, such as ease of application, confined delivery and improved patient compliance and comfort. One example of an injectable biomaterial is alginate, a natural polymer typically extracted from brown seaweeds and have been extensively investigated and used for many biomedical applications. The most common method to achieve alginate gelation and crosslinking is through the exchange of sodium ions from guluronic acid units with divalent cations like calcium. The ionic crosslinking can be obtained through external gelation, by dripping an alginate solution into a solution containing calcium ions, such as calcium chloride, under mild conditions. Alginate microbeads, due to their extracellular matrix-like features, are an excellent choice to be used has cell carrier, and for that reason have an enormous potential in the context of tissue regeneration. In this workshop, students will have the opportunity to get familiar with alginate as cell delivery system and to produce microbeads with different size and composition.

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Microalgae as a Nutraceutical in the Preparation of Yogurt


From 14:00 to 15:30

Instructor: Narcisa Bandarra

Western diets are poor in healthy polyunsaturated fatty acids, such as docosahexaenoic acid. Since microalgae Aurantiochytrium sp. is rich in docosahexaenoic acid, a functional food based on lean yogurt and this microalga was created. In this workshop, there will be an opportunity to learn about how the microalga was obtained, the in vitro digestion model, and the determination and characterization of the lipids present in the yogurt.

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3D Bioprinting for Tissue Engineering and Regenerative Engineering


From 14:00 to 16:30

Instructor: Ruben Pereira

This Workshop seeks to provide the fundamental concepts and cutting-edge advances on bioprinting strategies currently explored to create 3D constructs for tissue repair and in vitro tissue models for drug screening and personalized medicine. A special focus will be given on the different steps involved in the design of biofunctional bioinks capable of regulating the cell response within 3D microenvironments. Examples of bioconjugation reactions for biomaterial functionalization and crosslinking schemes for hydrogel crosslinking will be provided, along with the impact of microenvironmental cues on the biological function of bioprinted constructs. Finally, it will also provide insight in the translational challenges encountered in 3D bioprinting.

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Fitting the complexity of in vivo microenvironments into in vitro microfluidic chips


From 14:00 to 15:30

Instructor: Estrela Neto / Francisco Conceicao

"Learning about microfluidic devices and their advantages for studying cell-cell and cell-matrix interactions within their appropriate niche. By Dr. Estrela Neto and Francisco Conceicao, researchers at i3S." In this workshop, you will have an overview of the potential use of microfluidic technology in the biological sciences. How can such a small device replicate complex in vivo microenvironments? Moreover, you will contact with different designs of microfluidic devices and correlate with their physiological purpose. You will learn how to assemble them and even test different liquid flow inside the chips.

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Virtual Visit - Fraunhofer AICOS


From 14:00 to 15:00

The Research Center for Assistive Information and Communication Solutions - Fraunhofer Portugal AICOS - was founded in 2009, following a partnership between the Fraunhofer Society (Fraunhofer-Gesellschaft), the Foundation for Science and Technology and the University of Porto (UP). From its first industry research contract to its first product on the market, Fraunhofer Portugal AICOS has evolved significantly, raising notoriety, and growing its team in size as well as in diversity of competences. From its debut with 12 employees, AICOS now has two offices in Portugal (Porto and Lisbon) employing circa 100 people, of which 80 are highly qualified researchers. With a client portfolio from a broad range of areas, such as health, agriculture, retail or energy, Fraunhofer Portugal AICOS has consolidated competences in: Human-Centred Design; Intelligent Systems; and Connected Things.

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Computational Neuroscience: simulating neuronal dynamics in a computer


From 15:00 to 17:00

Instructor: Paulo Aguiar

Models play a fundamental role in science and this is particularly relevant in neuroscience. A powerful tool to understand the highly complex dynamics of neuronal activity is to carry out experiments in silico, using computational models. With these computational models, new ideas can be explored and hypotheses can be tested just as with in vitro or in vivo settings. This hands-on workshop will give the fundamental concepts on how to simulate biophysically realistic neurons in a computer and how to query and assess neuronal properties. We will use the NEURON simulation environment, a well-established tool freely available to the scientific community, to run detailed in silico experiments. Two models will be created and explored: a single cell model to analyse action potential initiation and conduction, and a small neuronal circuit model to analyse communication between neurons.

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Getting Started with BITalino


From 17:00 to 18:30

Instructor: Hugo Silva

Low-cost computing platforms, such as the Arduino and Raspberry Pi had a transformational role in the way people learn, experiment, and create imaginative solutions to outstanding problems that can benefit from embedded systems. Within biomedical engineering, physiological sensing is increasingly capacitated with analogous albeit specialised resources, the added value of which is being recognised by policymakers and practitioners across different disciplines. As a result, low-cost and open source tools for physiological sensing are providing new opportunities for biomedical engineers at different states of their careers, ranging from their first years of study to research and/or professional practice. During this workshop you will learn how to benefit from low-cost hardware and open source software to boost learning, research, and rapid prototyping in the field of biomedical engineering. Particular emphasis will be given to BITalino, which is the equivalent to the Arduino for biomedical sensing. We will review the basic concepts, show multiple projects and applications involving physiological sensing, explore modular wireless biosignal acquisition systems for real time data acquisition, and become familiar with useful software resources.

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Production of biofuels from microalgae


From 17:00 to 18:30

Instructor: Luis Costa

In this workshop, participants will learn about microalgae, how they can be connected to biofuels production and the various production pathways from microalgae to biofuels. Case studies of R&D projects targeting production of biofuels from algae will be presented, some of them involving A4F - Algae for future. The status of the microalgae sector and the current trends in the algae to biofuels sector will be discussed.

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